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Rio Play Store. Rio Play Store: Find your mobile apps, games, and lifestyle free, low cost, fast.The non-linear dynamics underlying the production of the pandemic (H1N1) 2009 seasonal A/Brisbane/59/2007 virus in Egypt.
To investigate the non-linear dynamics underlying the origin of the pandemic (H1N1) 2009 seasonal A/Brisbane/59/2007 virus in Egypt, the phenotype of the temporally evolving A/Brisbane/59/2007 population was studied. A mixed-effects model was developed to describe the observed changes in virus quasispecies throughout the evolution process. Our results suggest that the change in the virus quasispecies occurred through a non-linear process and a population bottleneck was occurring at the third passage at 10 days post-infection. Importantly, another bottleneck at 13 days post-infection was observed, suggesting another bottleneck or selection process. Moreover, our study suggests that the selection occurred at the amino acid position 136, which is a residue located in the viral neuraminidase protein. These data provide critical information for the development of a new class of antiviral drugs targeting the viral neuraminidase protein.An in vitro method to assess the neurotoxicity of ultrafine particles.
Large particulate matter (PM) air pollution is a major public health concern worldwide. Yet ultrafine particles (UFP), defined as particles with diameter smaller than 100 nm, are much smaller and thus expected to be more toxic than PM10 or PM2.5. Despite growing interest, there are currently no methods available to directly evaluate UFP toxicity. Herein, we developed an in vitro human neuroblastoma cell line (SHSY-5Y) model and assessed the toxicity of UFP in three different modes: cytotoxicity, oxidative stress, and apoptosis. We determined that the SHSY-5Y cells were viable up to 24 h after exposure to two different commercial grade UFP particles. By contrast, we found statistically significant increases in oxidative stress after 24 and 48 h of exposure to UFP, consistent with dose-dependent cell death in all three UFP groups. Surprisingly, we also observed a statistically significant dose-dependent increase in apoptosis (up to 24 h) only in the two highest UFP dose groups. Our cell model can serve as a useful predictive tool to assess the toxicity of UFP in future studies.Q:

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